Fig. 7. Dose-response of Rb⁺ influx, [K]_i, and Hb_c ODs in CR AgNPs-treated RBCs from human cord blood. Cord blood obtained at MVH was subjected to the procedures described in Materials and Methods and Fig. 1. During the flux period, RBCs for the CONT and NPS conditions were incubated with AgNPs at the following concentrations: 30, 60, 90, 120 and 150 µgmL^-1, at 37 °C for 60 min. In the control, identical volume of water instead of AgNPs was added for each concentration of AgNPs while maintaining constant osmolality along the concentration range. See legend to Fig. 3. for further details. Rb⁺ influx, [K]_i and Hb_c ODs were determined as described in Materials and Methods. Values are means ± S.D. Paired t-test was used to determine the statistical difference between NPS vs CONT. A) ***p<0.0001, n = 3 for Rb⁺ influx, B) ***p<0.0001, n = 3 and **p<0.001, n = 3) for [K]_i concentration, and C) ***p <0.0001, n = 3 for Hb_c ODs.